Analysis of Aldehydes in Beer Using Solid-Phase Microextraction with On-Fiber Derivatization and Gas Chromatography/Mass Spectrometry
- 28 October 2003
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 51 (24), 6941-6944
- https://doi.org/10.1021/jf034410t
Abstract
A new, fast, sensitive, and solventless extraction technique was developed in order to analyze beer carbonyl compounds. The method was based on solid-phase microextraction with on-fiber derivatization. A derivatization agent, O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBOA), was absorbed onto a divinyl benzene/poly(dimethylsiloxane) 65-μm fiber and exposed to the headspace of a vial with a beer sample. Carbonyl compounds selectively reacted with PFBOA, and the oximes formed were desorbed into a gas chromatograph injection port and quantified by mass spectrometry. This method provided very high reproducibility and linearity. When it was used for the analysis of aged beers, nine aldehydes were detected: 2-methylpropanal, 2-methylbutanal, 3-methylbutanal, pentanal, hexanal, furfural, methional, phenylacetaldehyde, and (E)-2-nonenal. Keywords: Aldehydes; beer analysis; derivatization; SPME; GC/MSThis publication has 5 references indexed in Scilit:
- Sampling and Determination of Formaldehyde Using Solid-Phase Microextraction with On-Fiber DerivatizationAnalytical Chemistry, 1998
- Analysis of aldehydes and ketones from beer as O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine derivativesTalanta, 1994
- FLAVOUR STABILITY OF ALES BREWED USING HYDROLYSED MAIZE SYRUP-SENSORY AND ANALYTICAL ASPECTSJournal of the Institute of Brewing, 1983
- USE OF GRAPHIC LINEAR SCALES TO MEASURE RATES OF STALING IN BEERJournal of Food Science, 1979
- 0-(2,3,4,5,6-Pentafluorobenzyl)Hydroxylamine Hydrochloride as a Sensitive Derivatizing Agent for the Electron Capture Gas Liquid Chromatographic Analysis of Keto SteroidsJournal of Chromatographic Science, 1975