H 2 O 2 -responsive liposomal nanoprobe for photoacoustic inflammation imaging and tumor theranostics via in vivo chromogenic assay
Top Cited Papers
- 8 May 2017
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 114 (21), 5343-5348
- https://doi.org/10.1073/pnas.1701976114
Abstract
Abnormal H2O2 levels are closely related to many diseases, including inflammation and cancers. Herein, we simultaneously load HRP and its substrate, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), into liposomal nanoparticles, obtaining a Lipo@HRP&ABTS optical nanoprobe for in vivo H2O2-responsive chromogenic assay with great specificity and sensitivity. In the presence of H2O2, colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of H2O2 down to submicromolar concentrations. Using Lipo@HRP&ABTS as an H2O2-responsive nanoprobe, we could accurately detect the inflammation processes induced by LPS or bacterial infection in which H2O2 is generated. Meanwhile, upon systemic administration of this nanoprobe we realize in vivo photoacoustic imaging of small s.c. tumors (∼2 mm in size) as well as orthotopic brain gliomas, by detecting H2O2 produced by tumor cells. Interestingly, local injection of Lipo@HRP&ABTS further enables differentiation of metastatic lymph nodes from those nonmetastatic ones, based on their difference in H2O2 contents. Moreover, using the H2O2-dependent strong NIR absorbance of Lipo@HRP&ABTS, tumor-specific photothermal therapy is also achieved. This work thus develops a sensitive H2O2-responsive optical nanoprobe useful not only for in vivo detection of inflammation but also for tumor-specific theranostic applications.Keywords
Funding Information
- Ministry of Science and Technology of the People's Republic of China (2016YFA0201200)
- National Natural Science Foundation of China (51525203)
This publication has 42 references indexed in Scilit:
- In vivo imaging of hydrogen peroxide production in a murine tumor model with a chemoselective bioluminescent reporterProceedings of the National Academy of Sciences of the United States of America, 2010
- Hypoxia, inflammation, and the tumor microenvironment in metastatic diseaseCancer and Metastasis Reviews, 2010
- In Vivo Photoacoustic Tomography of Chemicals: High-Resolution Functional and Molecular Optical Imaging at New DepthsChemical Reviews, 2010
- Golden carbon nanotubes as multimodal photoacoustic and photothermal high-contrast molecular agentsNature Nanotechnology, 2009
- Specific Targeting of Brain Tumors with an Optical/Magnetic Resonance Imaging Nanoprobe across the Blood-Brain BarrierCancer Research, 2009
- Carbon nanotubes as photoacoustic molecular imaging agents in living miceNature Nanotechnology, 2008
- Membrane transport of hydrogen peroxideBiochimica et Biophysica Acta (BBA) - Biomembranes, 2006
- Aptamers evolved from live cells as effective molecular probes for cancer studyProceedings of the National Academy of Sciences of the United States of America, 2006
- American Society of Clinical Oncology Guideline Recommendations for Sentinel Lymph Node Biopsy in Early-Stage Breast CancerJournal of Clinical Oncology, 2005
- Inflammation, Atherosclerosis, and Coronary Artery DiseaseThe New England Journal of Medicine, 2005