Cas-analyzer: an online tool for assessing genome editing results using NGS data
Open Access
- 24 August 2016
- journal article
- research article
- Published by Oxford University Press (OUP) in Bioinformatics
- Vol. 33 (2), 286-288
- https://doi.org/10.1093/bioinformatics/btw561
Abstract
Genome editing with programmable nucleases has been widely adopted in research and medicine. Next generation sequencing (NGS) platforms are now widely used for measuring the frequencies of mutations induced by CRISPR-Cas9 and other programmable nucleases. Here, we present an online tool, Cas-Analyzer, a JavaScript-based implementation for NGS data analysis. Because Cas-Analyzer is completely used at a client-side web browser on-the-fly, there is no need to upload very large NGS datasets to a server, a time-consuming step in genome editing analysis. Currently, Cas-Analyzer supports various programmable nucleases, including single nucleases and paired nucleases. Free access at http://www.rgenome.net/cas-analyzer/. Supplementary data are available at Bioinformatics online.Keywords
Funding Information
- Korea Healthcare technology R&D Project, Ministry for Health & Welfare Affairs (HI16C1012)
- S.B. and Institute for Basic Science (IBS-R021-D1)
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