A rapid assay for peroxidase activity
- 1 December 1966
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 101 (3), 582-583
- https://doi.org/10.1042/bj1010582
Abstract
1. Peroxidase has been assayed by a chronometric method involving the coupled reaction of ascorbic acid with the product of the enzymic action on benzidine. 2. Measurements of the activities of horseradish and tea peroxidase by this and two other methods, involving respectively pyrogallol and o-dianisidine, are compared. 3. It is claimed that the chronometric method is relatively simple, rapid and accurate. 4. The method can be used in the presence of polyphenol oxidases.Keywords
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