Quantitative analysis of myofilament protein phosphorylation in small cardiac biopsies
- 9 October 2007
- journal article
- research article
- Published by Wiley in Proteomics – Clinical Applications
- Vol. 1 (10), 1285-1290
- https://doi.org/10.1002/prca.200600891
Abstract
Phosphorylation of cardiac myofilament proteins represents one of the main post-translational mechanisms that regulate cardiac pump function. Human studies are often limited by the amount of available tissue as biopsies taken during cardiac catheterization weigh only 1 mg (dry weight). Similarly, investigation of time- (or dose-) dependent changes in protein phosphorylation in animal studies is often hampered by tissue availability. The present study describes quantitative analysis of phosphorylation status of multiple myofilament proteins by 2-DE and Pro-Q® Diamond stained gradient gels using minor amounts (˜0.5 mg dry weight) of human and pig cardiac tissue.Keywords
Funding Information
- Netherlands Organisation for Scientifc Research-2002
- Netherlands Heart Foundation (2000T042, 2005B220)
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