Dielectrophoresis of DNA: Quantification by impedance measurements
- 1 June 2010
- journal article
- research article
- Published by AIP Publishing in Biomicrofluidics
- Vol. 4 (2), 022803
- https://doi.org/10.1063/1.3430550
Abstract
Dielectrophoretic properties of DNA have been determined by measuring capacitance changes between planar microelectrodes. DNA sizes ranged from 100 bp to 48 kbp, DNA concentrations from below 0.1 to 70 μ g / ml . Dielectrophoretic spectra exhibited maximum response around 3 kHz and 3 MHz. The strongest response was found for very long DNA (above 10 kbp) and for short 100 bp fragments, which corresponds to the persistence length of DNA. The method allows for an uncomplicated, automatic acquisition of the dielectrophoretic properties of submicroscopical objects without the need for labeling protocols or optical accessibility.Keywords
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