In Vivo Muscle Protein Synthesis Rates in the American Lobster Homarus Americanus During the Moult Cycle and in Response to 20-Hydroxyecdysone

Abstract

Simultaneous measurements of in vivo rates of protein synthesis (Ks) in claw, leg and abdominal muscles were made in the American lobster Homarus americanus at three stages of the moult cycle. Ks values are significantly elevated during the premoult (stage D2-D3) and fall during the intermoult (stage C4) periods in all three muscles. Postmoult (stage A/B) levels are not significantly elevated above intermoult levels. Intermoult levels are between 0.3 and 0.4 % protein synthesized per day. In the premoult animals, the ribosomal activity (milligrams protein synthesized per microgram RNA per day) of the claw, abdominal and leg muscles is elevated three- to fivefold. The claw muscle maintains an elevated ribosomal activity into the postmoult stage whereas, by this stage, that of the other muscle tissues has fallen to intermoult levels. The RNA/protein ratios of the three muscle groups from intermoult, premoult and postmoult animals do not show any significant differences. 18S ribosomal RNA levels fluctuate slightly, with no consistent pattern over the moult cycle. In vivo injection of premoult concentrations of 20-hydroxyecdysone (20-HE) into intermoult lobsters results in elevated Ks values and ribosomal activity for the muscles after 3 days. RNA/protein ratios remain constant in the muscles in response to injections of 20-HE in vivo. In vitro preparations of leg muscle treated with 20-HE did not show similar elevated rates of protein synthesis.