Comparison of mechanical and electrical activity and interstitial cells of Cajal in urinary bladders from wild-type andW/Wvmice
- 1 January 2009
- journal article
- research article
- Published by Wiley in British Journal of Pharmacology
- Vol. 156 (2), 273-283
- https://doi.org/10.1111/j.1476-5381.2008.00006.x
Abstract
W/W(v) and wild-type murine bladders were studied to determine whether the W/W(v) phenotype, which causes a reduction in, but not abolition of, tyrosine kinase activity, is a useful tool to study the function of bladder interstitial cells of Cajal (ICC). Immunohistochemistry, tension recordings and microelectrode recordings of membrane potential were performed on wild-type and mutant bladders. Wild-type and W/W(v) detrusors contained c-Kit- and vimentin-immunopositive cells in comparable quantities, distribution and morphology. Electrical field stimulation evoked tetrodotoxin-sensitive contractions in wild-type and W/W(v) detrusor strips. Atropine reduced wild-type responses by 50% whereas a 25% reduction occurred in W/W(v) strips. The atropine-insensitive component was blocked by pyridoxal-5-phosphate-6-azophenyl-2',4'-disulphonic acid in both tissue types. Wild-type and W/W(v) detrusors had similar resting membrane potentials of -48 mV. Spontaneous electrical activity in both tissue types comprised action potentials and unitary potentials. Action potentials were nifedipine-sensitive whereas unitary potentials were not. Excitatory junction potentials were evoked by single pulses in both tissues. These were reduced by atropine in wild-type tissues but not in W/W(v) preparations. The atropine-insensitive component was abolished by pyridoxal-5-phosphate-6-azophenyl-2',4'-disulphonic acid in both preparations. Bladders from W/W(v) mice contain c-Kit- and vimentin-immunopositive ICC. There are similarities in the electrical and contractile properties of W/W(v) and wild-type detrusors. However, significant differences were found in the pharmacology of the responses to neurogenic stimulation with an apparent up-regulation of the purinergic component. These findings indicate that the W/W(v) strain may not be the best model to study ICC function in the bladder.This publication has 49 references indexed in Scilit:
- Guide to Receptors and Channels (GRAC), 3rd editionBritish Journal of Pharmacology, 2008
- Cholinergic-induced Ca2+ signaling in interstitial cells of Cajal from the guinea pig bladderAmerican Journal of Physiology-Renal Physiology, 2008
- Purinergic receptors in gastrointestinal inflammationAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2008
- Kit mutants and gastrointestinal physiologyJournal Of Physiology-London, 2006
- Calcium currents in interstitial cells from the guinea-pig bladderBJU International, 2006
- Alterations in purinergic and cholinergic components of contractile responses of isolated detrusor contraction in a rat model of partial bladder outlet obstructionBJU International, 2006
- Electrical Characteristics of Suburothelial Cells Isolated From the Human BladderJournal of Urology, 2004
- Regional variation in contribution of myenteric and intramuscular interstitial cells of Cajal to generation of slow waves in mouse gastric antrumJournal Of Physiology-London, 2002
- P2X RECEPTORS AND THEIR ROLE IN FEMALE IDIOPATHIC DETRUSOR INSTABILITYJournal of Urology, 2002
- ALTERATIONS IN CHOLINERGIC AND PURINERGIC SIGNALING IN A MODEL OF THE OBSTRUCTED BLADDERJournal of Urology, 2001