Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol.
- 1 April 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 81 (7), 2117-2121
- https://doi.org/10.1073/pnas.81.7.2117
Abstract
The ability of purified Rous sarcoma virus transforming gene product, pp60v-src, to phosphorylate phosphatidylinositol and diacylglycerol was investigated. Phosphatidylinositol was phosphorylated to form both mono- and diphosphorylated derivatives. 1,2-Diacylglycerol was phosphorylated to form phosphatidic acid. These activities showed the same thermolability and the same sensitivity to inhibitors as shown by the casein kinase activity of pp60v-src. In addition, when serum-starved chicken embryo fibroblasts transformed by a virus mutant temperature-sensitive for transformation were shifted from the nonpermissive to permissive temperature, an increase of 50-100% in the labeling of phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate, and phosphatidic acid was observed, as compared to uninfected cells.Keywords
This publication has 36 references indexed in Scilit:
- Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphateNature, 1983
- The effect of quercetin on the phosphorylatio activity of the Rous sarcoma virus transforming gene product in vitro and in vivoJBIC Journal of Biological Inorganic Chemistry, 1983
- Three glycolytic enzymes are phosphorylated at tyrosine in cells transformed by Rous sarcoma virusNature, 1983
- Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene productCell, 1980
- Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virusCell, 1980
- Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistryCell, 1979
- Phosphorylation-Dephosphorylation of EnzymesAnnual Review of Biochemistry, 1979
- Immunofluorescence on avian sarcoma virus-transformed cells: Localization of the src gene productCell, 1979
- Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoproteinCell, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970