Resolution of Racemic Amino Acids by Gas Chromatography on Optically Active Stationary Phases

Abstract

Amino acid enantiomers can be resolved by GC on optically active stationary phases such as N-TFA-L-valyl-L-valine cyclohexyl ester and N-TFA-L-phenylalanyl-L-leucine cyclohexyl ester. TFA-amino acid isopropyl esters have been thus far the most suitable derivatives for this purpose, allowing resolution of higher volatile amino acids. PFP amino acid isopropyl esters have retention times which are up to 47% shorter and give separation of the lower volatile amino acids without a significant loss in the resolution factors. Due to this fact it is now possible to resolve seventeen pairs of the common protein amino acids.