Group-Reactive ELISAs for Detecting Antibodies to African Horsesickness and Equine Encephalosis Viruses in Horse, Donkey, and Zebra Sera
Open Access
- 1 January 1993
- journal article
- research article
- Published by SAGE Publications in Journal of Veterinary Diagnostic Investigation
- Vol. 5 (1), 3-7
- https://doi.org/10.1177/104063879300500102
Abstract
Group-reactive enzyme-linked immunosorbent assays (ELISAs) were developed to selectively detect antibodies to African horsesickness virus (AHSV) and equine encephalosis virus (EEV), 2 orbiviruses that infect equids. In indirect ELISA, guinea pig antisera to all known AHSV or EEV serotypes recognized immobilized AHSV serotype 3 or EEV Cascara, respectively. Antisera from naturally infected animals did not cross-react with their respective heterologous viruses. The ELISA was used in parallel with the complement fixation (CF) and agar gel immunodiffusion tests to detect antibodies in sera from animals in the field. The ELISA distinguished among those that contained antibodies to AHSV, EEV, or both viruses and was useful with sera that did not yield results in CF tests because of anticomplementary activity. Zebra and donkeys, both potential subclinical carrier animals in Africa, contained AHSV or EEV antibodies. Some sera reacted with 1 of the 2 orbiviruses, whereas others reacted with both. The ELISA can be used in projected epidemiological studies in which many serum samples must be assayed.Keywords
This publication has 12 references indexed in Scilit:
- The use of ELISA for the detection of African horse sickness viruses in Culicoides midgesJournal of Virological Methods, 1991
- A serogroup specific enzyme-linked immunosorbent assay for the detection and identification of African horse sickness virusesJournal of Virological Methods, 1991
- Purification and characterization of the major group-specific core antigen VP7 of bluetongue virus synthesized by a recombinant baculovirusJournal of General Virology, 1990
- An indirect sandwich ELISA utilising F(ab′)2 fragments for the detection of African horsesickness virusJournal of Virological Methods, 1990
- A competitive ELISA for the detection of group-specific antibodies to African horse sickness virusEpidemiology and Infection, 1990
- Characterization and cloning of the African horsesickness virus genomeJournal of General Virology, 1990
- Laboratory Diagnosis of African Horse Sickness: Comparison of Serological Techniques and Evaluation of Storage Methods of Samples for Virus IsolationJournal of Veterinary Diagnostic Investigation, 1990
- Isolation of a capsid protein of bluetongue virus that induces a protective immune response in sheepVirology, 1987
- Reduction in non-specific binding in enzyme immunoassays using case in hydrolysate in serum diluentsJournal of Immunological Methods, 1985
- Techniques for Hemagglutination and Hemagglutination-Inhibition with Arthropod-Borne VirusesThe American Journal of Tropical Medicine and Hygiene, 1958