Mapping of Antigenic Sites on the Nucleocapsid Protein of the Severe Acute Respiratory Syndrome Coronavirus
Open Access
- 1 November 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (11), 5309-5314
- https://doi.org/10.1128/jcm.42.11.5309-5314.2004
Abstract
Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests.Keywords
This publication has 29 references indexed in Scilit:
- Profile of Antibodies to the Nucleocapsid Protein of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Probable SARS PatientsClinical and Vaccine Immunology, 2004
- Assessment of Immunoreactive Synthetic Peptides from the Structural Proteins of Severe Acute Respiratory Syndrome CoronavirusClinical Chemistry, 2003
- The Genome Sequence of the SARS-Associated CoronavirusScience, 2003
- Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory SyndromeScience, 2003
- A complete sequence and comparative analysis of a SARS-associated virus (Isolate BJ01)Chinese Science Bulletin, 2003
- Is CD46 the cellular receptor for measles virus?Virus Research, 1997
- Mouse hepatitis virus spike and nucleocapsid proteins expressed by adenovirus vectors protect mice against a lethal infectionJournal of General Virology, 1993
- Induction of anti-viral immune responses by immunization with recombinant-DNA encoded avian coronavirus nucleocapsid proteinVaccine, 1992
- A semi‐empirical method for prediction of antigenic determinants on protein antigensFEBS Letters, 1990
- Ribonucleoprotein-like Structures from Coronavirus ParticlesJournal of General Virology, 1978