The protein interaction network of a taxis signal transduction system in a Halophilic Archaeon
Open Access
- 1 January 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 12 (1), 1-20
- https://doi.org/10.1186/1471-2180-12-272
Abstract
The taxis signaling system of the extreme halophilic archaeon Halobacterium (Hbt.) salinarum differs in several aspects from its model bacterial counterparts Escherichia coli and Bacillus subtilis. We studied the protein interactions in the Hbt. salinarum taxis signaling system to gain an understanding of its structure, to gain knowledge about its known components and to search for new members. The interaction analysis revealed that the core signaling proteins are involved in different protein complexes and our data provide evidence for dynamic interchanges between them. Fifteen of the eighteen taxis receptors (halobacterial transducers, Htrs) can be assigned to four different groups depending on their interactions with the core signaling proteins. Only one of these groups, which contains six of the eight Htrs with known signals, shows the composition expected for signaling complexes (receptor, kinase CheA, adaptor CheW, response regulator CheY). From the two Hbt. salinarum CheW proteins, only CheW1 is engaged in signaling complexes with Htrs and CheA, whereas CheW2 interacts with Htrs but not with CheA. CheY connects the core signaling structure to a subnetwork consisting of the two CheF proteins (which build a link to the flagellar apparatus), CheD (the hub of the subnetwork), two CheC complexes and the receptor methylesterase CheB. Based on our findings, we propose two hypotheses. First, Hbt. salinarum might have the capability to dynamically adjust the impact of certain Htrs or Htr clusters depending on its current needs or environmental conditions. Secondly, we propose a hypothetical feedback loop from the response regulator to Htr methylation made from the CheC proteins, CheD and CheB, which might contribute to adaptation analogous to the CheC/CheD system of B. subtilis.Keywords
This publication has 93 references indexed in Scilit:
- A proteome-wide protein interaction map for Campylobacter jejuniGenome Biology, 2007
- Determinants of chemoreceptor cluster formation in Escherichia coliMolecular Microbiology, 2006
- Large-scale identification of protein–protein interaction of Escherichia coli K-12Genome Research, 2006
- A uniform proteomics MS/MS analysis platform utilizing open XML file formatsMolecular Systems Biology, 2005
- Analyzing yeast protein–protein interaction data obtained from different sourcesNature Biotechnology, 2002
- A novel mode of sensory transduction in archaea: binding protein-mediated chemotaxis towards osmoprotectants and amino acidsThe EMBO Journal, 2002
- Mechanism of CheA Protein Kinase Activation in Receptor Signaling ComplexesBiochemistry, 1999
- Deletion Analysis of thecheOperon in the ArchaeonHalobacterium salinariumJournal of Molecular Biology, 1996
- Transformation methods for halophilic archaebacteriaCanadian Journal of Microbiology, 1989
- Solute concentrations within cells of halophilic and non-halophilic bacteriaBiochimica et Biophysica Acta, 1962