Evolution of AbGRI2-0, the Progenitor of the AbGRI2 Resistance Island in Global Clone 2 of Acinetobacter baumannii
Open Access
- 1 March 2016
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 60 (3), 1421-1429
- https://doi.org/10.1128/aac.02662-15
Abstract
A320, isolated in the Netherlands in 1982 and also known as RUH134, is the earliest available multiply antibiotic-resistant (MAR) Acinetobacter baumannii isolate belonging to global clone 2 (GC2) and is the reference strain for this clone. The draft genome sequence of A320 was used to investigate the original location and configuration of the IS 26 -bounded AbGRI2 resistance island found in current GC2 isolates. PCR mapping and sequencing were used to order contigs composing the resistance islands. A320 contains two IS 26 -bounded resistance islands, AbGRI2-0a and AbGRI2-0b, of 7.8 kb and 25.4 kb, respectively. Together they contain bla TEM , aacC1 , aadA1 , sul1 , catA1 , and aphA1b genes, which confer resistance to antibiotics used clinically in the 1970s, as well as an incomplete mercury resistance module. Tracking the continuity of the chromosome and the target site duplications revealed that the two resistance islands were originally together as AbGRI2-0, an island of 32.4 kb, and were subsequently separated via an IS 26 -mediated intramolecular inversion that reversed the orientation of 1.54 Mb of the chromosome and duplicated an IS 26 . A320 contains an ancestral form of AbGRI2, and the original insertion site of the AbGRI2 island was identified. Many of the AbGRI2 versions present in the completed GC2 genomes can be derived from it via the variant AbGRI2-1. IS 26 -mediated inversions have also played a part in forming AbGRI2-0, and, upon reversal, large regions of AbGRI2-0 are identical to parts of AbaR0, the ancestral version of the AbaR islands present in GC1 isolates. This indicates a common source.Keywords
This publication has 36 references indexed in Scilit:
- Genome Sequence of Acinetobacter baumannii Strain A1, an Early Example of Antibiotic-Resistant Global Clone 1Microbiology Resource Announcements, 2015
- Carbapenem and amikacin resistance on a large conjugative Acinetobacter baumannii plasmidJournal of Antimicrobial Chemotherapy, 2014
- A conjugative plasmid carrying the carbapenem resistance gene blaOXA-23 in AbaR4 in an extensively resistant GC1 Acinetobacter baumannii isolateJournal of Antimicrobial Chemotherapy, 2014
- Dissemination of 16S rRNA Methylase ArmA-Producing Acinetobacter baumannii and Emergence of OXA-72 Carbapenemase Coproducers in JapanAntimicrobial Agents and Chemotherapy, 2014
- Identification of a marker for two lineages within the GC1 clone of Acinetobacter baumannii.Journal of Antimicrobial Chemotherapy, 2013
- Tn6167, an antibiotic resistance island in an Australian carbapenem-resistant Acinetobacter baumannii GC2, ST92 isolateJournal of Antimicrobial Chemotherapy, 2012
- Antibiotic resistance islands in A320 (RUH134), the reference strain for Acinetobacter baumannii global clone 2Journal of Antimicrobial Chemotherapy, 2011
- Genomic Analysis of the Multidrug-Resistant Acinetobacter baumannii Strain MDR-ZJ06 Widely Spread in ChinaAntimicrobial Agents and Chemotherapy, 2011
- Evolution of IncP-1α Plasmids by Acquisition of Antibiotic and Mercuric Ion Resistance TransposonsMicrobial Drug Resistance, 2011
- Diversity of aminoglycoside-resistance genes and their association with class 1 integrons among strains of pan-European Acinetobacter baumannii clonesJournal of Medical Microbiology, 2004