Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria
- 1 December 2001
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 19 (12), 1162-1167
- https://doi.org/10.1038/nbt1201-1162
Abstract
Mobile group II introns can be retargeted to insert into virtually any desired DNA target. Here we show that retargeted group II introns can be used for highly specific chromosomal gene disruption in Escherichia coli and other bacteria at frequencies of 0.1–22%. Furthermore, the introns can be used to introduce targeted chromosomal breaks, which can be repaired by transformation with a homologous DNA fragment, enabling the introduction of point mutations. Because of their wide host range, mobile group II introns should be useful for genetic engineering and functional genomics in a wide variety of bacteria.This publication has 28 references indexed in Scilit:
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