Mutation detection by denaturing gradient gel electrophoresis (DGGE)
- 1 January 1994
- journal article
- research article
- Published by Hindawi Limited in Human Mutation
- Vol. 3 (2), 83-94
- https://doi.org/10.1002/humu.1380030202
Abstract
The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease‐causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically amplified DNA. The technique is based on the migration of double‐stranded DNA molecules through polyacrylamide gels containing linearly increasing concentrations of a denaturing agent. In this review DGGE and the several modifications of the original protocol are presented. Moreover, its applications in human molecular genetics are summarized together with a preliminary comparison with other mutation detection technologies such as chemical cleavage, RNase protection, and single‐strand conformation polymorphism.Keywords
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