Disentangling conformational states of macromolecules in 3D-EM through likelihood optimization
Top Cited Papers
- 10 December 2006
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 4 (1), 27-29
- https://doi.org/10.1038/nmeth992
Abstract
Although three-dimensional electron microscopy (3D-EM) permits structural characterization of macromolecular assemblies in distinct functional states, the inability to classify projections from structurally heterogeneous samples has severely limited its application. We present a maximum likelihood–based classification method that does not depend on prior knowledge about the structural variability, and demonstrate its effectiveness for two macromolecular assemblies with different types of conformational variability: the Escherichia coli ribosome and Simian virus 40 (SV40) large T-antigen.This publication has 13 references indexed in Scilit:
- Fast maximum-likelihood refinement of electron microscopy imagesBioinformatics, 2005
- Maximum-likelihood Multi-reference Refinement for Electron Microscopy ImagesJournal of Molecular Biology, 2005
- XMIPP: a new generation of an open-source image processing package for electron microscopyJournal of Structural Biology, 2004
- Structure determination of macromolecular assemblies by single-particle analysis of cryo-electron micrographsCurrent Opinion in Structural Biology, 2004
- Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM datasetJournal of Structural Biology, 2004
- Molecular dynamics of protein complexes from four-dimensional cryo-electron microscopyJournal of Structural Biology, 2004
- Experimental Verification of Conformational Variation of Human Fatty Acid Synthase as Predicted by Normal Mode AnalysisStructure, 2004
- Large T antigen on the simian virus 40 origin of replication: a 3D snapshot prior to DNA replicationThe EMBO Journal, 2003
- Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation processThe EMBO Journal, 2002
- A Maximum-Likelihood Approach to Single-Particle Image RefinementJournal of Structural Biology, 1998