DNA topoisomerase I is involved in both repression and activation of transcription
- 16 September 1993
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 365 (6443), 227-232
- https://doi.org/10.1038/365227a0
Abstract
Reconstituted transcription reactions containing the seven general transcription factors, in addition to RNA polymerase II, respond poorly to transcriptional activators. Two factors, Dr2 and ACF, necessary for high levels of transcription in response to an activator have been identified. ACF can enhance basal and activated transcription. Dr2 represses basal transcrip-tion, but this can be overcome by transcriptional activators or TFIIA. Dr2 is human DNA topoiso-merase I. The DNA relaxation activity of topoisomerase I is dispensable for transcriptional repression. The effect of Dr2 is specific for TATA-box-containing promoters and is mediated by the TATA-binding protein.Keywords
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