On the pH‐optimum of activity and stability of proteins
- 1 June 2010
- journal article
- research article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 78 (12), 2699-2706
- https://doi.org/10.1002/prot.22786
Abstract
Biological macromolecules evolved to perform their function in specific cellular environment (subcellular compartments or tissues); therefore, they should be adapted to the biophysical characteristics of the corresponding environment, one of them being the characteristic pH. Many macromolecular properties are pH dependent, such as activity and stability. However, only activity is biologically important, while stability may not be crucial for the corresponding reaction. Here, we show that the pH‐optimum of activity (the pH of maximal activity) is correlated with the pH‐optimum of stability (the pH of maximal stability) on a set of 310 proteins with available experimental data. We speculate that such a correlation is needed to allow the corresponding macromolecules to tolerate small pH fluctuations that are inevitable with cellular function. Our findings rationalize the efforts of correlating the pH of maximal stability and the characteristic pH of subcellular compartments, as only pH of activity is subject of evolutionary pressure. In addition, our analysis confirmed the previous observation that pH‐optimum of activity and stability are not correlated with the isoelectric point, pI, or with the optimal temperature. Proteins 2010.Keywords
This publication has 65 references indexed in Scilit:
- Evidence for the adaptation of protein pH-dependence to subcellular pHBMC Biology, 2009
- BRENDA, AMENDA and FRENDA the enzyme information system: new content and tools in 2009Nucleic Acids Research, 2009
- Structural model and functional significance of pH-dependent talin–actin binding for focal adhesion remodelingProceedings of the National Academy of Sciences of the United States of America, 2008
- Probing the pH-Dependent Prepore to Pore Transition of Bacillus anthracis Protective Antigen with Differential Oxidative Protein FootprintingBiochemistry, 2008
- Structural Insights into the Mechanism of pH-dependent Ligand Binding and Release by the Cation-dependent Mannose 6-Phosphate ReceptorPublished by Elsevier BV ,2008
- LocateP: Genome-scale subcellular-location predictor for bacterial proteinsBMC Bioinformatics, 2008
- pH-dependent Binding of the Epsin ENTH Domain and the AP180 ANTH Domain to PI(4,5)P2-containing BilayersJournal of Molecular Biology, 2007
- Advantages of combined transmembrane topology and signal peptide prediction--the Phobius web serverNucleic Acids Research, 2007
- BRENDA, AMENDA and FRENDA: the enzyme information system in 2007Nucleic Acids Research, 2007
- Prediction of protein cellular attributes using pseudo‐amino acid compositionProteins-Structure Function and Bioinformatics, 2001