Clonal origin of pituitary adenomas
- 1 November 1990
- journal article
- Published by Journal of Neurosurgery Publishing Group (JNSPG) in Journal of Neurosurgery
- Vol. 73 (5), 731-735
- https://doi.org/10.3171/jns.1990.73.5.0731
Abstract
Benign pituitary adenomas are among the most common neurosurgical tumors and account for a diversity of clinical syndromes due to their hormone content and release. To determine whether these tumors arise from a single cell or multiple cells, the authors studied X chromosome inactivation in deoxyribonucleic acid (DNA) isolated from pituitary adenomas in women. Tumors of three different hormonal subtypes were examined. One tumor contained cells immunoreactive for prolactin and human growth hormone; one tumor contained foci immunoreactive for the beta-subunits of luteinizing hormone and follicle-stimulating hormone; and the third tumor had no immunoreactive prolactin, human growth hormone, beta-subunits of thyroid-stimulating hormone, luteinizing hormone, or follicle-stimulating hormone, or the alpha-subunit. Analysis of the DNA revealed that, in each of the three pituitary tumors, one X chromosome was active in all cells and one X chromosome was inactive, indicating that each of these tumors was monoclonal in origin. It is concluded that clinically evident pituitary tumors arise from a genetic mutation in a single cell.Keywords
This publication has 8 references indexed in Scilit:
- Monoclonality and Abnormal Parathyroid Hormone Genes in Parathyroid AdenomasNew England Journal of Medicine, 1988
- Hormone production in clinically nonfunctioning pituitary adenomasJournal of Neurosurgery, 1987
- Loss of genes on chromosome 22 in tumorigenesis of human acoustic neuromaNature, 1986
- Use of Restriction Fragment Length Polymorphisms to Determine the Clonal Origin of Human TumorsScience, 1985
- Differential methylation of hypoxanthine phosphoribosyltransferase genes on active and inactive human X chromosomes.Proceedings of the National Academy of Sciences of the United States of America, 1984
- A three-allele restriction-fragment-length polymorphism at the hypoxanthine phosphoribosyltransferase locus in man.Proceedings of the National Academy of Sciences of the United States of America, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975