Life-on-a-chip
- 1 November 2003
- journal article
- review article
- Published by Springer Science and Business Media LLC in Nature Reviews Microbiology
- Vol. 1 (2), 158-164
- https://doi.org/10.1038/nrmicro755
Abstract
Mechanistic studies of cellular processes are usually carried out with large populations of cells. However, parameters that are measured as averages of large populations can be misleading. For instance, an apparently linear response to a signal could, in fact, reflect an increasing number of cells in the population that have switched from 'off' to 'on', rather than a graded increase in response by all the cells. At present, the study of single cells is challenging, but new technologies mean it might soon be a reality.Keywords
This publication has 46 references indexed in Scilit:
- Noise in eukaryotic gene expressionNature, 2003
- Intrinsic and extrinsic contributions to stochasticity in gene expressionProceedings of the National Academy of Sciences of the United States of America, 2002
- Stochastic Gene Expression in a Single CellScience, 2002
- Stochastic Kinetic Analysis of Developmental Pathway Bifurcation in Phage λ-Infected Escherichia coli CellsGenetics, 1998
- Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populationsProceedings of the National Academy of Sciences of the United States of America, 1997
- Stochastic mechanisms in gene expressionProceedings of the National Academy of Sciences of the United States of America, 1997
- Environmental regulation of the fim switch controlling type 1 fimbrial phase variation in Escherichia coli K-12: effects of temperature and mediaJournal of Bacteriology, 1993
- Growth conditions mediate differential transcription of fim genes involved in phase variation of type 1 piliJournal of Bacteriology, 1992
- Distribution of suboptimally induced β-d-galactosidase in Escherichia coli: The enzyme content of individual cellsJournal of Molecular Biology, 1973
- ENZYME INDUCTION AS AN ALL-OR-NONE PHENOMENONProceedings of the National Academy of Sciences of the United States of America, 1957