Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection
- 13 April 2007
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of Materials Chemistry
- Vol. 17 (24), 2561-2565
- https://doi.org/10.1039/b617402g
Abstract
A novel, efficient DNA delivery and transfection agent has been developed by chemically attaching short chain polyethyleneimine (PEI) molecules (M.W. = 423 and 1800) to the surface of composite iron oxide nanoparticles (250 nm in diameter). These particles are not only capable of delivering DNA but also can be driven by an external magnetic field. During DNA binding experiments, a remarkably low N : P ratio of via simple electrostatic interactions. This new attachment strategy allows the use of smaller amounts of PEI with potential reduction in toxic side-effects.Keywords
This publication has 15 references indexed in Scilit:
- Gene therapy progress and prospects: magnetic nanoparticle-based gene deliveryGene Therapy, 2006
- Synthesis of magnetic nanoparticles and their application to bioassaysAnalytical and Bioanalytical Chemistry, 2006
- Magnetic nanoparticles for drug deliveryDrug Development Research, 2006
- Medical application of functionalized magnetic nanoparticlesJournal of Bioscience and Bioengineering, 2005
- Gene transfer with modified polyethyleniminesThe Journal of Gene Medicine, 2004
- Applications of magnetic nanoparticles in biomedicineJournal of Physics D: Applied Physics, 2003
- The Magnetofection Method: Using Magnetic Force to Enhance Gene DeliveryBiological Chemistry, 2003
- Improved Method of Recombinant AAV2 Delivery for Systemic Targeted Gene TherapyMolecular Therapy, 2002
- A Powerful Nonviral Vector for In Vivo Gene Transfer into the Adult Mammalian Brain: PolyethylenimineHuman Gene Therapy, 1996
- A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.Proceedings of the National Academy of Sciences of the United States of America, 1995