Comparison of Disc Diffusion Methods for the Detection of Extended-Spectrum Beta Lactamase-Producing Enterobacteriaceae
Open Access
- 29 January 2011
- journal article
- research article
- Published by Scientific Scholar in Journal of Laboratory Physicians
- Vol. 3 (01), 033-036
- https://doi.org/10.4103/0974-2727.78561
Abstract
Background: Resistance to broad-spectrum β lactams, mediated by extended-spectrum β lactamases (ESBLs), is an increasing problem world wide. This resistance poses problems for in vitro testing and reporting. Increased prevalence of ESBLs among Enterobacteriaceae creates a great need for laboratory testing methods that will accurately identify their presence. Materials and Methods: During the study, the Enterobacteriaceae isolated were tested for the presence of ESBL by the National Committee for Clinical Laboratory Standards (NCCLS) screening test, Jarlier double disc synergy (approximation) test (DDST) and NCCLS phenotypic confirmatory test (PCT), and compared their efficiency in detection. Results: A total of 313 Enterobacteriaceae were isolated and tested for the presence of ESBL. NCCLS PCT identified 200 (63.89%) as ESBL producers and DDST identified 176 (56.23%), with a P-value of <0.001. Among the screening agents, ceftazidime had a better sensitivity (89.49%) and specificity (95.74%). Conclusions: Close monitoring of the susceptibility pattern of isolates and careful spacing with specific discs can identify many ESBL producers. Ceftazidime has a better sensitivity and specificity as a screening agent. A combination of different tests can be useful for accurate identification.Keywords
This publication has 17 references indexed in Scilit:
- Evaluation of Methods for AmpC Beta-Lactamase in Gram Negative Clinical Isolates from Tertiary Care HospitalsIndian Journal of Medical Microbiology, 2005
- Prevalence of clinical strains resistant to various beta-lactams in a tertiary care hospital in India.2004
- EXTENDED SPECTRUM β-LACTAMASES IN URINARY ISOLATES OF ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE - PREVALENCE AND SUSCEPTIBILITY PATTERN IN A TERTIARY CARE HOSPITALIndian Journal of Medical Microbiology, 2004
- EXTENDED SPECTRUM β-LACTAMASES (ESBL) – AN EMERGING THREAT TO CLINICAL THERAPEUTICSIndian Journal of Medical Microbiology, 2004
- PREVALENCE OF EXTENDED SPECTRUM β -LACTAMASE PRODUCING KLEBSIELLA PNEUMONIAE IN A TERTIARY CARE HOSPITALIndian Journal of Medical Microbiology, 2004
- Evaluation of the NCCLS Extended-Spectrum β-Lactamase Confirmation Methods for Escherichia coli with Isolates Collected during Project ICAREJournal of Clinical Microbiology, 2003
- Prevalence of extended spectrum beta lactamase producing gram negative bacteria in a tertiary care hospital.2002
- Extended-Spectrum β-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance ThreatClinical Microbiology Reviews, 2001
- Detection of extended-spectrum -lactamases in members of the family Enterobacteriaceae: comparison of the MAST DD test, the double disc and the Etest ESBLJournal of Antimicrobial Chemotherapy, 2000
- Extended-spectrum beta-lactamasesAntimicrobial Agents and Chemotherapy, 1989