CD8+ T cells mediate the regenerative PTH effect in hPDL cells via Wnt10b signaling
- 17 October 2016
- journal article
- research article
- Published by SAGE Publications in Innate Immunity
- Vol. 22 (8), 674-681
- https://doi.org/10.1177/1753425916669417
Abstract
It was the aim of the present investigation to examine whether the stimulating effect of parathyroid hormone (PTH) on human periodontal ligament (hPDL) cell proliferation and differentiation would be enhanced by hPDL/T-cell interaction involving Wnt10b signaling as a mediating pathway. hPDL cells were cultured from healthy premolar tissues of three adolescent orthodontic patients and exposed to PTH(1-34) in monocultures or co-cultures with CD8+ T cells. At harvest, proliferation, alkaline phosphatase-specific activity (ALP), and osteocalcin production were determined by immunofluorescence cytochemistry, real-time PCR, biochemical assay, and ELISA. Wnt10b signaling was analyzed by the use of a specific WNT10b neutralizing antibody. PTH(1-34) stimulation of T cells significantly increased Wnt10b expression and production. Wnt10b exposure of hPDL cells enhanced proliferation and differentiation. PDL cells co-cultured with T cells showed a Wnt10b-dependent regulation of proliferation and differentiation parameters. The addition of a Wnt10b-neutralizing Ab to the co-culture medium resulted in a significant inhibition of the PTH(1-34) effect on proliferation, ALP-specific activity, and osteocalcin protein expression. Our findings provide novel insight into the mechanism of action of PTH on hPDL cells and establish the interplay of T cells and hPDL cells via the Wnt10b pathway as a modulating factor for the anabolic properties of the hormone in periodontal regeneration.Keywords
This publication has 34 references indexed in Scilit:
- Treatment with intermittent PTH increases Wnt10b production by T cells in osteoporotic patientsOsteoporosis International, 2015
- High-mobility group box protein-1 released by human-periodontal ligament cells modulates macrophage migration and activity in vitroInnate Immunity, 2013
- T cells: Critical bone regulators in health and diseaseBone, 2010
- The immune system and boneArchives of Biochemistry and Biophysics, 2010
- T Lymphocytes Amplify the Anabolic Activity of Parathyroid Hormone through Wnt10b SignalingCell Metabolism, 2009
- PTH and PTHrP signaling in osteoblastsCellular Signalling, 2009
- Chronic Elevation of Parathyroid Hormone in Mice Reduces Expression of Sclerostin by Osteocytes: A Novel Mechanism for Hormonal Control of OsteoblastogenesisEndocrinology, 2005
- Anabolic actions of PTH (1-34): Use of a novel tissue engineering model to investigate temporal effects on boneBone, 2005
- Effect of intermittent PTH administration in the periodontitis-associated bone loss in ovariectomized ratsArchives of Oral Biology, 2005
- Parathyroid Hormone Protects against Periodontitis-associated Bone LossJournal of Dental Research, 2003