Antibodies to histone (h2a–h2b)–dna complexes in the absence of antibodies to double‐stranded dna or to (h2a–h2b) complexes are more sensitive and specific for scleroderma‐related disorders than for lupus

Abstract

Objective. To assess the role of antibodies to histones H2A, H2B, and anti–double‐stranded DNA which form (H2A–H2B)–DNA complexes in patients with scleroderma‐related disorders. Methods. Antihistone antibodies were measured, by enzyme immunoassay, in 26 patients with scleroderma‐related disorders, 100 patients with systemic lupus erythematosus (SLE), and 24 patients with rheumatoid arthritis. Results. Antibodies to histone (H2A–H2B)–DNA complex were more commonly seen in patients with scleroderma‐related disorders than in those with SLE (P < 0.0001). Conclusion. Scleroderma‐related disorders should be included among conditions in which various types of antihistone antibodies are produced. A hypothesis to account for this finding is discussed.