Volatile anesthetic modulation of oligomerization equilibria in a hexameric model peptide
- 16 November 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 578 (1-2), 140-144
- https://doi.org/10.1016/j.febslet.2004.10.087
Abstract
To determine if occupancy of interfacial pockets in oligomeric proteins by volatile anesthetic molecules can allosterically regulate oligomerization equilibria, variants of a three-helix bundle peptide able to form higher oligomers were studied with analytical ultracentrifugation, hydrogen exchange and modeling. Halothane shifted the oligomerization equilibria towards the oligomer only in a mutation predicted to create sufficient volume in the hexameric pocket. Other mutations at this residue, predicted to create a too small or too polar pocket, were unaffected by halothane. Inhaled anesthetic modulation of oligomerization interactions is a novel and potentially generalizable biophysical basis for some anesthetic actions.Keywords
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