Activation of substance P receptors leads to membrane potential responses in cultured astrocytes
- 1 January 1989
- Vol. 2 (3), 155-160
- https://doi.org/10.1002/glia.440020304
Abstract
Cultured astrocytes from rat cortex and spinal cord responded with different types of membrane potential changes upon brief (10 seconds) applications of the natural neurokinin agonists substance P and neurokinin A. The most prominent type of response was a long-lasting membrane depolarization. In some cells, an initial rapid depolarization followed by a partial repolarization preceded the slow depolarizing event. Few astrocytes responded with a hyperpolarization of the membrane. Selective agonists at the NK-1 receptive site, substance P-methyl ester (SP-OME) and septide, mimicked the response to the natural neurokinins as did DiMe-C7, a selective NK-3 receptor agonist. A putative neurokinin antagonist, (D-Arg1,D-Pro2,D-Trp7,9,Leu11)SP (DADPDT) partially blocked membrane potential responses induced by substance P, SP-OME, septide, DiMe-C7, and NKA. The authors conclude that astrocytes express NK-1 and NK-3 receptors, which upon activation affect the electrical properties of these cells.Keywords
This publication has 22 references indexed in Scilit:
- Tachykinins modulate multiple ionic conductances in voltage-clamped rat spinal dorsal horn neuronsRegulatory Peptides, 1988
- Selective presence of a high density of NK-1 receptors on astrocytes from the rat brain stem in primary cultureRegulatory Peptides, 1988
- Does substance P act as a pain transmitter?Trends in Pharmacological Sciences, 1987
- Substance P-immunoreactive astrocytes related to deep white matter and striatal blood vessels in human brainBrain Research, 1986
- [pGlu6, Pro9]SP6-11, a selective agonist for the substance P P-receptor subtypeJournal of Medicinal Chemistry, 1986
- Substance P augments a persistent slow inward calcium-sensitive current in voltage-clamped spinal dorsal horn neurons of the ratBrain Research, 1986
- Tissue selectivity of substance P alkyl esters: Suggesting multiple receptorsEuropean Journal of Pharmacology, 1983
- Substance P Analog, DiMe-C7: Evidence for Stability in Rat Brain and Prolonged Central ActionsScience, 1982
- Intrathecal morphine inhibits substance P release from mammalian spinal cord in vivoNature, 1980
- Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.The Journal of cell biology, 1980