Lack of intratumoral heterogeneity in DNA ploidy pattern of hepatocellular carcinoma
- 30 November 1993
- journal article
- Published by Elsevier BV in Gastroenterology
- Vol. 105 (5), 1449-1454
- https://doi.org/10.1016/0016-5085(93)90150-b
Abstract
From biological and clinical perspectives, it is important to clarify tumor heterogeneity. This study was aimed to investigate whether or not intratumoral heterogeneity of DNA ploidy pattern exists in hepatocellular carcinoma (HCC). Using fresh materials resected from 31 untreated patients, DNA ploidy was analyzed at different sites of the same HCC by means of flow cytometry. The tumor size ranged from 1.7 to 25.0 cm. There was no case in which euploid and aneuploid HCCs coexisted in the same tumor. The DNA ploidy pattern was euploid in 15 and aneuploid in 16 instances. Of 15 euploid tumors, the areas analyzed were all diploid in 12 and tetraploid in 2 but diploid/tetraploid in 1. Among 16 aneuploid tumors, the DNA indices (DI) at different sites were similar in 9, but apparently, different aneuploid subclones coexisted in 7 cases. The incidence of DI heterogeneity in aneuploid HCCs was similar between small (< 5 cm) and large (> or = 5 cm) tumors; 3 of 7 (42.9%) versus 4 of 9 (44.4%). It is assumed that euploid and aneuploid HCCs develop in their own ploidy pattern and that the evolution of aneuploid subpopulations from euploid HCC is rare, but new aneuploid subclones can evolve from aneuploid HCC due to increased instability of its karyotype.Keywords
This publication has 23 references indexed in Scilit:
- Comparison between diploid and aneuploid hepatocellular carcinomas: A flow cytometric studyBritish Journal of Surgery, 1992
- Prediction of relapse or survival after resection in human hepatomas by DNA flow cytometry.JCI Insight, 1992
- Intratumoral variations in dna distribution patterns in mammary adenocarcinomasCytometry, 1989
- DNA clonal heterogeneity of hepatocellular carcinoma demonstrated by Feulgen‐DNA analysisLiver International, 1987
- Clinical and biological significance of aneuploidy in human tumours.Journal of Clinical Pathology, 1984
- Convention on nomenclature for DNA cytometryCytometry, 1984
- Flow-cytometric demonstration of tumour-cell subpopulations with different DNA content in human colo-rectal carcinomaEuropean Journal of Cancer (1965), 1979
- The morphology of cirrhosis. Recommendations on definition, nomenclature, and classification by a working group sponsored by the World Health Organization.Journal of Clinical Pathology, 1978
- The Clonal Evolution of Tumor Cell PopulationsScience, 1976
- Primary carcinoma of the liver.A study of 100 cases among 48,900 necropsiesCancer, 1954