Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-Based Microbial Community Composition
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- 1 December 2000
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 66 (12), 5488-5491
- https://doi.org/10.1128/aem.66.12.5488-5491.2000
Abstract
A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.Keywords
This publication has 26 references indexed in Scilit:
- Bacterial Community Structure and Physiological State within an Industrial Phenol Bioremediation SystemApplied and Environmental Microbiology, 2000
- Stable-isotope probing as a tool in microbial ecologyNature, 2000
- Comparison of different methods for the isolation and purification of total community DNA from soilJournal of Microbiological Methods, 1999
- Identification of novel bacterial lineages as active members of microbial populations in a freshwater sediment using a rapid RNA extraction procedure and RT-PCRMicrobiology, 1999
- Community analysis of ammonia-oxidising bacteria, in relation to oxygen availability in soils and root-oxygenated sediments, using PCR, DGGE and oligonucleotide probe hybridisationFEMS Microbiology Ecology, 1998
- Community analysis of ammonia-oxidising bacteria, in relation to oxygen availability in soils and root-oxygenated sediments, using PCR, DGGE and oligonucleotide probe hybridisationFEMS Microbiology Ecology, 1998
- Extraction of ribosomal RNA and genomic DNA from soil for studying the diversity of the indigenous bacterial community.Journal of Microbiological Methods, 1998
- Microbial Evolution, Diversity, and Ecology: A Decade of Ribosomal RNA Analysis of Uncultivated MicroorganismsMicrobial Ecology, 1998
- Direct extraction and purification of rRNA for ecological studiesApplied and Environmental Microbiology, 1993
- [2] Creating a ribonuclease-free environmentMethods in Enzymology, 1987