Bright Ideas for Chemical Biology
- 1 March 2008
- journal article
- review article
- Published by American Chemical Society (ACS) in ACS Chemical Biology
- Vol. 3 (3), 142-155
- https://doi.org/10.1021/cb700248m
Abstract
Small-molecule fluorescent probes embody an essential facet of chemical biology. Although numerous compounds are known, the ensemble of fluorescent probes is based on a modest collection of modular “core” dyes. The elaboration of these dyes with diverse chemical moieties is enabling the precise interrogation of biochemical and biological systems. The importance of fluorescence-based technologies in chemical biology elicits a necessity to understand the major classes of small-molecule fluorophores. Here, we examine the chemical and photophysical properties of oft-used fluorophores and highlight classic and contemporary examples in which utility has been built upon these scaffolds.Keywords
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