Comparison of proliferative and multilineage differentiation potentials of cord matrix, cord blood, and bone marrow mesenchymal stem cells
Open Access
- 1 January 2010
- journal article
- research article
- Published by Medknow in Asian Journal of Transfusion Science
- Vol. 4 (1), 14-24
- https://doi.org/10.4103/0973-6247.59386
Abstract
Background: Hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) are the two widely studied and characterized adult stem cells. Thus far, MSCs were obtained from the bone marrow, which is a painful procedure. Therefore, MSCs from less common sources like cord blood, adipose tissue, tooth pulp, and so on, have been the subject of research. The purpose of this study is to explore the possibility of finding MSCs from a less controversial, easy, and abundant source, such as the umbilical cord, for potential regenerative medicine applications. Study Design and Methods: Five bone marrow samples (BM), seventy cord blood units (CB), and four umbilical cord matrix (CM) samples have been used for the study. Expanded MSCs were checked for biomarker expression by flow cytometry and were also checked for their differentiation to mesodermal and ectodermal lineages. Results: MSCs could be isolated from 100% BM and CM samples, as compared to only 6% of CB samples. The fold expansion of the mesenchymal stem cells observed in CB (CB-MSCs) was distinctly higher as compared to BM (BM-MSCs) and CM (CM-MSCs). MSCs isolated from all the three sources expressed a characteristic mesenchymal phenotype of CD45-/vWF-/CD14-/CD31-/CD73+/CD105+/SSEA4+/CD29+/CD44+/HLAABC+, whereas, the HLA DR was conspicuously absent in CM-MSCs and CB-MSCs. Although osteogenic, chondrogenic, and neural differentiation was observed in MSCs from all sources, adipogenic differentiation was observed only in BM-MSCs. Conclusion: CM-MSCs are a dependable source of an unlimited number of MSCs for autologous and allogenic use in regenerative medicine.Keywords
This publication has 19 references indexed in Scilit:
- Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose TissueThe International Journal of Cell Cloning, 2006
- Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord bloodExperimental Hematology, 2005
- Disparate Mesenchyme-Lineage Tendencies in Mesenchymal Stem Cells from Human Bone Marrow and Umbilical Cord BloodThe International Journal of Cell Cloning, 2005
- Growth Kinetics of Human Mesenchymal Stem Cells from Bone Marrow and Umbilical Cord BloodActa Haematologica, 2004
- Mesenchymal stem cells: clinical applications and biological characterizationThe International Journal of Biochemistry & Cell Biology, 2004
- Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult bloodExperimental Hematology, 2002
- Mesenchymal stem cells: Will they have a role in the clinic?Journal of Cellular Biochemistry, 2002
- Mesenchymal stem cells: heading into the clinicBone Marrow Transplantation, 2001
- Mesenchymal progenitor cells in human umbilical cord bloodBritish Journal of Haematology, 2000
- Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfectaNature Medicine, 1999