MicroRNA‐145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB
- 13 July 2012
- journal article
- Published by Wiley in FEBS Letters
- Vol. 586 (19), 3263-3270
- https://doi.org/10.1016/j.febslet.2012.06.048
Abstract
MicroRNAs (miRNAs) are a class of 21- to 25-nucleotide non-coding RNAs, some of which are important gene regulators involved in folliculogenesis. In this study, we used CCK-8, real-time PCR and Western blot assays to demonstrate that miR-145 inhibits mouse granulosa cell (mGC) proliferation. Combined with the results of luciferase reporter assays that studied the 3′-untranslated region of ACVRIB mRNA, these assays identified ACVRIB as a direct target of miR-145. The ectopic expression of miR-145 reduced the levels of both ACVRIB mRNA and protein and also interfered with activin-induced Smad2 phosphorylation. Altogether, this study revealed that miR-145 suppresses mGC proliferation by targeting ACVRIBThis publication has 35 references indexed in Scilit:
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