Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn's disease
Open Access
- 1 September 2012
- journal article
- Published by Taylor & Francis Ltd in Clinical and Experimental Gastroenterology
- Vol. 5, 173-186
- https://doi.org/10.2147/CEG.S33858
Abstract
Objectives: The aim of this study was to define the composition of the gut bacterial flora in Norwegian patients with early stage Crohn's disease (CD). Methods: By using a nonselective metagenomics approach, the general bacterial composition in mucosal biopsies from the ileum and the colon of five subjects, four patients with different phenotypes of CD, and one noninflammatory bowel disease control, was characterized. After partial 16S ribosomal RNA (rRNA) gene sequencing, BLAST homology searches for species identification and phylogenetic analysis were performed. Results: An overall biodiversity of 106 different bacterial operational taxonomic units (OTUs) was detected in the cloned libraries. Nearly all OTUs belonged to the phylae Bacteroidetes (42% in CD, 71% in the control) or Firmicutes (42% in CD, 28% in the control), except for some OTUs that belonged to the phylum Proteobacteria (15% in CD, 0% in the control) and a few OTUs that could not be assigned to a phylum (2% in CD, 1% in the control). Conclusion: Based on the high incidence of inflammatory bowel disease (IBD) in Norway, this pilot study represents a relevant determination of the gut microbiota in Norwegian patients compared to previous findings in other countries. The bacterial profile of Norwegian CD patients was found to be similar to that of CD patients in other countries. The findings do not support a particular bacterial composition as a predominant causative factor for the high incidence of IBD that exists in some countries.Keywords
This publication has 35 references indexed in Scilit:
- Enterotypes of the human gut microbiomeNature, 2011
- The Montreal classification of inflammatory bowel disease: controversies, consensus, and implicationsGut, 2006
- Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approachGut, 2006
- Molecular characterization of the bacteria adherent to human colorectal mucosaJournal of Applied Microbiology, 2006
- Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genesFEMS Microbiology Ecology, 2005
- Spatial Organization and Composition of the Mucosal Flora in Patients with Inflammatory Bowel DiseaseJournal of Clinical Microbiology, 2005
- High prevalence of adherent-invasive Escherichia coli associated with ileal mucosa in Crohn’s diseaseGastroenterology, 2004
- Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel diseaseGut, 2004
- Mucosa-Associated Bacteria in the Human Gastrointestinal Tract Are Uniformly Distributed along the Colon and Differ from the Community Recovered from FecesApplied and Environmental Microbiology, 2002
- Incidence of inflammatory bowel disease across Europe: is there a difference between north and south? Results of the European Collaborative Study on Inflammatory Bowel Disease (EC-IBD).Gut, 1996