Vasopressin mRNA in situ hybridization: localization and regulation studied with oligonucleotide cDNA probes in normal and Brattleboro rat hypothalamus.
- 1 August 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 82 (16), 5555-5559
- https://doi.org/10.1073/pnas.82.16.5555
Abstract
Hybridizable vasopressin mRNA may be quantitatively localized in situ in sections from rat hypothalamus. Radiolabeled oligonucleotide cDNA probes, synthesized by chemical and enzymatic means, provide strong hybridization in zones known to contain vasopressin cell bodies. Multiple single-stranded 32P-, 35S-, or 3H-labeled oligonucleotides demonstrate localized hybridization that increases as probes are lengthened from 8 to 75 bases. Competition studies, RNase experiments, anatomic localization, and use of multiple probes all support hybridization specificity. An approximate doubling of hybridizable mRNA in both supraoptic and paraventricular nuclei can be detected with dehydration of the animals. Hybridizable mRNA densities are virtually normal in hypothalamic nuclei of Brattleboro rats given free access to water. These methods can provide insight into regional mRNA dynamics and may reflect functional activity of peptidergic neurons.Keywords
This publication has 37 references indexed in Scilit:
- Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probesDevelopmental Biology, 1984
- Differential Responses to Osmotic Stress of Vasopressin-Neurophysin mRNA in Hypothalamic NucleiNeuroendocrinology, 1984
- Expression-cloning and sequence of a cDNA encoding human growth hormone-releasing factorNature, 1983
- Nucleotide sequences of cloned cDNAs for two types of bovine brain substance P precursorNature, 1983
- Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processingNature, 1983
- In SituHybridization Histochemistry: A Technique for the Study of Gene Expression in Single CellsDNA, 1983
- A monoclonal antibody to vasopressin: preparation, characterization, and application in immunocytochemistry.Journal of Histochemistry & Cytochemistry, 1982
- Brain Peptides as NeurotransmittersScience, 1980
- A new method for receptor autoradiography: [3H]Opioid receptors in rat brainBrain Research, 1979
- Localisation of cellular globin messenger RNA by in situ hybridisation to complementary DNAFEBS Letters, 1973