A New Black Aspergillus Species, A. vadensis , Is a Promising Host for Homologous and Heterologous Protein Production
Open Access
- 1 July 2004
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 70 (7), 3954-3959
- https://doi.org/10.1128/aem.70.7.3954-3959.2004
Abstract
A new species of the group of black aspergilli, Aspergillus vadensis , was analyzed for its potential as a host for homologous and heterologous protein production. Unlike the other black aspergilli, this strain does not acidify the culture medium when nitrate is the nitrogen source and only produces very low levels of extracellular proteases, mainly serine metalloproteases. The stability of A. tubingensis feruloyl esterase A (FaeA) was compared upon production in wild-type A. vadensis , A. tubingensis , and an A. niger strain in which the three main protease-encoding genes were disrupted. The production of FaeA in A. vadensis resulted in larger amounts of intact protein than production in A. tubingensis and was similar to production in an A. niger protease disruptant, confirming in vivo the low proteolytic activity of A. vadensis . The protoplast formation and transformation efficiencies of A. vadensis were much higher than those of A. niger . These characteristics make A. vadensis a very promising candidate for homologous, and possibly heterologous, protein production.Keywords
This publication has 18 references indexed in Scilit:
- Studies on the Production of Fungal Peroxidases in Aspergillus nigerApplied and Environmental Microbiology, 2000
- Expression of catalytic subunit of bovine enterokinase in the filamentous fungus Aspergillus nigerJournal of Biotechnology, 2000
- Quenched BODIPY Dye-Labeled Casein Substrates for the Assay of Protease Activity by Direct Fluorescence MeasurementAnalytical Biochemistry, 1997
- Aspergillus as a host for heterologous protein production: the problem of proteasesTrends in Biotechnology, 1997
- Disruption of Three Acid Proteases in Aspergillus Niger— Effects on Protease Spectrum, Intracellular Proteolysis, and Degradation of Target ProteinsEuropean Journal of Biochemistry, 1997
- Evaluation of RFLP analysis of the classification of selected black aspergilliMycological Research, 1997
- Regulation of secreted protein production by filamentous fungi: recent developments and perspectivesJournal of General Microbiology, 1993
- Commercial Levels of Chymosin Production by AspergillusBio/Technology, 1991
- Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulansCurrent Genetics, 1991
- Improvement of enzyme production in AspergillusAntonie van Leeuwenhoek, 1987