Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens
- 1 November 1988
- journal article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 76 (5), 767-774
- https://doi.org/10.1007/bf00303524
Abstract
Leaves of the in vitro grown potato cultivars 'Bintje', 'Berolina', 'Desiree', and 'Russet Burbank' were wounded and co-cultivated with Agrobacterium strains having chimeric bar and nptII genes on a disarmed T-DNA. Each leaf from these cultivars formed numerous calli on kanamycin-containing medium, and almost all calli regenerated shoots. For 'Russet Burbank', it was necessary to include AgNO3 in the medium to obtain efficient shoot regeneration. The transformed plants have one to a few copies of the T-DNA, show NPT-II and PAT activities, and are resistant to high doses of the commercial preparation of phospinotricin (glufosinate). Almost no somaclonal variation was detected in trans-genic plants.Keywords
This publication has 30 references indexed in Scilit:
- Inhibition of Somatic Embryogenesis in Tissue 2Medicago sativaby Aminoethoxyvinylglycine, Amino-oxyacetic acid, 2, 4-Dinitrophenol and Salicylic Acid at Concentrations which do not Inhibit Ethylene Biosynthesis and GrowthJournal of Experimental Botany, 1988
- Abscission: Ethylene and Light ControlPlant Physiology, 1987
- Antiethylene properties of AgNO3 and 2,5-norbornadiene in light and dark inVigna radiataJournal of Plant Growth Regulation, 1987
- A Simple and General Method for Transferring Genes into PlantsScience, 1985
- Biosynthesis of Ethylene from Methionine in Aminoethoxyvinylglycine-Resistant Avocado TissuePlant Physiology, 1982
- Variability of Protoplast‐derived Potato Clones1Crop Science, 1981
- Auxin-induced Ethylene Production and Its Inhibition by Aminoethyoxyvinylglycine and Cobalt IonPlant Physiology, 1979
- Production and Action of Ethylene in Senescing Leaf DiscsPlant Physiology, 1979
- Nutrient requirements of suspension cultures of soybean root cellsExperimental Cell Research, 1968
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962