Prenatal microbiological risk factors associated with preterm birth

Abstract

Objective To study the vaginal flora of pregnant women at 22–28 weeks gestation to determine whether the presence of specific micro-organisms is significantly associated with preterm birth and prelabour rupture of the membranes. Design A comprehensive descriptive prospective study of the vaginal micro-flora of women between 22–28 weeks gestation comparing those who gave birth preterm (Tri-chomonas vaginalis. Multiple logistic regression analysis was used to account for confounding obstetric and demographic variables. Setting The Queen Victoria Hospital, Adelaide, South Australia. Subjects 135 women who gave birth preterm compared to 651 women who gave birth at term. Main outcome measure Preterm birth and preterm prelabour rupture of membranes (PROM) Results The prevalence of Gardnerella vaginalis between 22–28 weeks was significantly higher in women who gave birth preterm compared to women who gave birth at term (23%vs 15%; multiple logistic regression odds ratio (OR) 1.8, 95% confidence intervals (CI) 1.01–3.2, PUreaplasma urealyticum was also found in a higher proportion of women who gave birth preterm (49% vs 32% OR 1.7, 95% CI 1.1–2.6, PU. urealyticum between 22–28 weeks, compared with 32% in the term group (OR 3.2, CI 1.7–6.1, fG. vaginalis was also significantly associated with preterm PROM (OR 2.7, CI 1.1–6.5, PE. coli, Klebsiella spp., Haemophilus spp., Staph. aureus) in the midtrimester was not predictive of preterm birth, but when these organisms were found in labour, they appeared to have been acquired later in the pregnancy. Conclusion Women carrying G. vaginalis or U. urealyticum during the midtrimester had nearly twice the risk of preterm birth, while women positive for U. urealyticum had more than a threefold risk of preterm PROM.