Recognition and repair of UV lesions in loop structures of duplex DNA by DASH-type cryptochrome
- 30 December 2008
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (52), 21023-21027
- https://doi.org/10.1073/pnas.0805830106
Abstract
DNA photolyases and cryptochromes (cry) form a family of flavoproteins that use light energy in the blue/UV-A region for the repair of UV-induced DNA lesions or for signaling, respectively. Very recently, it was shown that members of the DASH cryptochrome subclade repair specifically cyclobutane pyrimidine dimers (CPDs) in UV-damaged single-stranded DNA. Here, we report the crystal structure of Arabidopsis cryptochrome 3 with an in-situ-repaired CPD substrate in single-stranded DNA. The structure shows a binding mode similar to that of conventional DNA photolyases. Furthermore, CPD lesions in double-stranded DNA are bound and repaired with similar efficiency as in single-stranded DNA if the CPD lesion is present in a loop structure. Together, these data reveal that DASH cryptochromes catalyze light-driven DNA repair like conventional photolyases but lack an efficient flipping mechanism for interaction with CPD lesions within duplex DNA.This publication has 36 references indexed in Scilit:
- More Than a Repair Enzyme:Aspergillus nidulansPhotolyase-like CryA Is a Regulator of Sexual DevelopmentMolecular Biology of the Cell, 2008
- Cryptochrome mediates light-dependent magnetosensitivity in DrosophilaNature, 2008
- Discrimination of Class I Cyclobutane Pyrimidine Dimer Photolyase from Blue Light Photoreceptors by Single Methionine ResidueBiophysical Journal, 2008
- Trichoderma atroviride PHR1, a Fungal Photolyase Responsible for DNA Repair, Autoregulates Its Own PhotoinductionEukaryotic Cell, 2007
- Cryptochrome Signaling in Plants†Photochemistry and Photobiology, 2007
- Photoselected electron transfer pathways in DNA photolyaseProceedings of the National Academy of Sciences of the United States of America, 2007
- Crystal structure of cryptochrome 3 from Arabidopsis thaliana and its implications for photolyase activityProceedings of the National Academy of Sciences of the United States of America, 2006
- A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activityProceedings of the National Academy of Sciences of the United States of America, 2006
- Natural and Non‐natural Antenna Chromophores in the DNA Photolyase from Thermus ThermophilusChemBioChem, 2006
- The CCP4 suite: programs for protein crystallographyActa crystallographica. Section D, Structural biology, 1994