Morphology, Molecular Genetics, and Bioacoustics Support Two New Sympatric Xenophrys Toads (Amphibia: Anura: Megophryidae) in Southeast China
Open Access
- 8 April 2014
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 9 (4), e93075
- https://doi.org/10.1371/journal.pone.0093075
Abstract
Given their recent worldwide declines and extinctions, characterization of species-level diversity is of critical importance for large-scale biodiversity assessments and conservation of amphibians. This task is made difficult by the existence of cryptic species complexes, species groups comprising closely related and morphologically analogous species. The combination of morphology, genetic, and bioacoustic analyses permits robust and accurate species identification. Using these methods, we discovered two undescribed Xenophrys species, namely Xenophrys lini sp. nov. and Xenophrys cheni sp. nov. from the middle range of Luoxiao Mountains, southeast China. These two new species can be reliably distinguished from other known congeners by morphological and morphometric differences, distinctness in male advertisement calls, and substantial genetic distances (>3.6%) based on the mitochondrial 16s and 12s rRNA genes. The two new species, together with X. jinggangensis, are sympatric in the middle range of Luoxiao Mountains but may be isolated altitudinally and ecologically. Our study provides a first step to help resolve previously unrecognized cryptic biodiversity and provides insights into the understanding of Xenophrys diversification in the mountain complexes of southeast China.Keywords
This publication has 40 references indexed in Scilit:
- High levels of cryptic species diversity uncovered in Amazonian frogsProceedings Of The Royal Society B-Biological Sciences, 2011
- A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of extant frogs, salamanders, and caeciliansMolecular Phylogenetics and Evolution, 2011
- MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony MethodsMolecular Biology and Evolution, 2011
- DnaSP v5: a software for comprehensive analysis of DNA polymorphism dataBioinformatics, 2009
- Are we in the midst of the sixth mass extinction? A view from the world of amphibiansProceedings of the National Academy of Sciences of the United States of America, 2008
- jModelTest: Phylogenetic Model AveragingMolecular Biology and Evolution, 2008
- High level of cryptic species diversity revealed by sympatric lineages of Southeast Asian forest frogsBiology Letters, 2006
- Application of Phylogenetic Networks in Evolutionary StudiesMolecular Biology and Evolution, 2005
- Neighbor-Net: An Agglomerative Method for the Construction of Phylogenetic NetworksMolecular Biology and Evolution, 2003
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994