Integron-Locatedoxa-32Gene Cassette Encoding an Extended-Spectrum Variant of OXA-2 β-Lactamase fromPseudomonas aeruginosa
Open Access
- 1 February 2002
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 46 (2), 566-569
- https://doi.org/10.1128/aac.46.2.566-569.2002
Abstract
Pseudomonas aeruginosaclinical isolate CY-1, which was resistant to ceftazidime, harbored a conjugative ca. 250-kb plasmid that contained a class 1 integron with two gene cassettes encoding OXA-32, an OXA-2- type β-lactamase, and the aminoglycoside acetyltransferase AAC(6′)Ib9. OXA-32 differed from OXA-2 by an Leu169Ile amino acid substitution (class D numbering). Site-directed mutagenesis established that Ile169 is responsible for resistance to ceftazidime but not to cefotaxime.Keywords
This publication has 21 references indexed in Scilit:
- OXA-28, an Extended-Spectrum Variant of OXA-10 β-Lactamase fromPseudomonas aeruginosaand Its Plasmid- and Integron-Located GeneAntimicrobial Agents and Chemotherapy, 2001
- OXA-24, a Novel Class D β-Lactamase with Carbapenemase Activity in an Acinetobacter baumannii Clinical StrainAntimicrobial Agents and Chemotherapy, 2000
- Sequence Analysis of ARI-1, a Novel OXA β-Lactamase, Responsible for Imipenem Resistance in Acinetobacter baumannii 6B92Antimicrobial Agents and Chemotherapy, 2000
- Carbapenems as inhibitors of OXA-13, a novel, integron-encoded β-lactamase in Pseudomonas aeruginosaMicrobiology, 1998
- Gene cassettes: a new class of mobile elementMicrobiology, 1995
- A spontaneous point mutation i theaac(6′)-lb′gene results in altered substrate specificity of aminoglycoside 6′-N-acetyltransferase of aPseudomonas fluorescensstrainFEMS Microbiology Letters, 1994
- Phylogeny of LCR‐1 and OXA‐5 with class A and class D β‐lactamasesMolecular Microbiology, 1992
- Comparison of the sequences of class A beta-lactamases and of the secondary structure elements of penicillin-recognizing proteinsAntimicrobial Agents and Chemotherapy, 1991
- New plasmid-mediated oxacillin-hydrolyzing β-lactamase in Pseudomonas aeruginosaJournal of Antimicrobial Chemotherapy, 1986
- Sequence of the OXA2 β‐lactamase: comparison with other penicillin‐reactive enzymesFEBS Letters, 1985