Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
Open Access
- 13 September 2011
- journal article
- research article
- Published by Hindawi Limited in Oxidative Medicine and Cellular Longevity
- Vol. 2011, 1-8
- https://doi.org/10.1155/2011/282739
Abstract
The word autophagy broadly refers to the cellular catabolic processes that lead to the removal of damaged cytosolic proteins or cell organelles through lysosomes. Although autophagy is often observed during programmed cell death, it may also serve as a cell survival mechanism. Accumulation of reactive oxygen species within tissues and cells induces various defense mechanisms or programmed cell death. It has been shown that, besides inducing apoptosis, oxidative stress can also induce autophagy. To date, however, the regulation of autophagy in response to oxidative stress remains largely elusive and poorly understood. Therefore, the present study was designed to examine the ratio between oxidative stress and autophagy in macrophages after oxidant exposure (AAPH) and to investigate the ultrastructural localization of beclin-1, a protein essential for autophagy, under basal and stressful conditions. Our data provide evidence that oxidative stress induces autophagy in macrophages. We demonstrate, for the first time by immunoelectron microscopy, the subcellular localization of beclin-1 in autophagic cells.Keywords
This publication has 42 references indexed in Scilit:
- The regulation of autophagy – unanswered questionsJournal of Cell Science, 2011
- Negative regulation of autophagyCell Death & Differentiation, 2010
- Linking ER Stress to Autophagy: Potential Implications for Cancer TherapyInternational Journal of Cell Biology, 2010
- Antitumor effect of matrine in human hepatoma G2 cells by inducing apoptosis and autophagyWorld Journal of Gastroenterology, 2010
- Autophagy, Redox Signaling, and Ventricular RemodelingAntioxidants and Redox Signaling, 2009
- LPS-induced autophagy is mediated by oxidative signaling in cardiomyocytes and is associated with cytoprotectionAmerican Journal of Physiology-Heart and Circulatory Physiology, 2009
- Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinaseProceedings of the National Academy of Sciences of the United States of America, 2008
- Toll-like receptors control autophagyThe EMBO Journal, 2008
- Mechanisms of Cell Death in Oxidative StressAntioxidants and Redox Signaling, 2007
- Autophagy Contributes to Caspase-independent Macrophage Cell DeathPublished by Elsevier BV ,2006