Chlamydia trachomatis Inclusions Induce Asymmetric Cleavage Furrow Formation and Ingression Failure in Host Cells
- 1 December 2011
- journal article
- Published by Informa UK Limited in Molecular and Cellular Biology
- Vol. 31 (24), 5011-5022
- https://doi.org/10.1128/mcb.05734-11
Abstract
Chlamydia trachomatis infection has been suggested to induce host genome duplication and is linked to increased risks of cervical cancer. We describe here the mechanism by which Chlamydia causes a cleavage furrow defect that consistently results in the formation of multinucleated host cells, a phenomenon linked to tumorigenesis. Host signaling proteins essential for cleavage furrow initiation, ingression, and stabilization are displaced from one of the prospective furrowing cortices after Chlamydia infection. This protein displacement leads to the formation of a unique asymmetrical, unilateral cleavage furrow in infected human cells. The asymmetrical distribution of signaling proteins is caused by the physical presence of the Chlamydia inclusion at the cell equator. By using ingested latex beads, we demonstrate that the presence of a large vacuole at the cell equator is sufficient to cause furrow ingression failure and can lead to multinucleation. Interestingly, internalized latex beads of similar size do not localize to the cell equator as efficiently as Chlamydia inclusions; moreover, inhibition of bacterial protein synthesis with antibiotic reduces the frequency at which Chlamydia localizes to the cell equator. Together, these results suggest that Chlamydia effectors are involved in strategic positioning of the inclusion during cell division.Keywords
This publication has 55 references indexed in Scilit:
- Chlamydia trachomatis Infection Causes Mitotic Spindle Pole Defects Independently from its Effects on Centrosome AmplificationTraffic, 2011
- The Transforming Parasite Theileria Co-opts Host Cell Mitotic and Central Spindles to Persist in Continuously Dividing CellsPLoS Biology, 2010
- Mechanisms of chromosome behaviour during mitosisNature Reviews Molecular Cell Biology, 2010
- Centrosome abnormalities during aChlamydia trachomatisinfection are caused by dysregulation of the normal duplication pathwayCellular Microbiology, 2009
- Plk1 Self-Organization and Priming Phosphorylation of HsCYK-4 at the Spindle Midzone Regulate the Onset of Division in Human CellsPLoS Biology, 2009
- Polo-Like Kinase 1 Directs Assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF Complex to Initiate Cleavage Furrow FormationPLoS Biology, 2009
- Phagosome maturation: going through the acid testNature Reviews Molecular Cell Biology, 2008
- Lifeact: a versatile marker to visualize F-actinNature Methods, 2008
- Anillin: a pivotal organizer of the cytokinetic machineryBiochemical Society Transactions, 2008
- Mechanisms of host cell exit by the intracellular bacteriumChlamydiaProceedings of the National Academy of Sciences of the United States of America, 2007