Multicenter evaluation of a whole-blood filter that saves platelets
- 20 July 2004
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 44 (8), 1197-1203
- https://doi.org/10.1111/j.1537-2995.2004.03350.x
Abstract
Whole-blood (WB) leukoreduction filters in current use retain the majority of PLTs. A new whole-blood filter, which retains significantly fewer of the PLTs (or saves PLTs [WB-SP]), has been developed. The performance characteristics of the WB-SP filter have been evaluated in a multicenter study. A total of 617 units of WB was collected into quadruple bag sets with an integrated WB-SP filter, leukoreduced, and processed into leukoreduced RBCs (LR-RBC), plasma (LR-PL), and buffy coats (LR-BC) from which, pooled, leukoreduced, PLT concentrates (LR-PCs) were produced. Recovery, yield, and residual WBCs were assessed in prepared blood components. The median residual WBC number in the LR-RBCs was 0.05 x 10(6) (range, <0.05-3.8), exceeding 1 x 10(6) in 0.6 percent of the units. Median Hb content in LR-RBC was 50 g (range, 34-72), reflecting a final RBC recovery of 81 +/- 6 percent. The median WBC content of the LR-PC was 0.05 x 10(6) (range, <0.05-0.28), with none exceeding 1 x 10(6). The median PLT content of the LR-PC, per individual donation, was 6.4 x 10(10) (range, 4.1-10.7), representing a final recovery of 62 +/- 10 percent. The mean FVIII activity was 104 +/- 25 percent and 83 +/- 11 percent in plasma separated from fresh or overnight stored WB, respectively. Use of the WB-SP filter makes it possible to obtain three leukoreduced blood components with only one filtration step. The WB-SP filter showed good leukoreduction performance and recovery of all blood components including PLTs.Keywords
This publication has 21 references indexed in Scilit:
- The effect of prestorage WBC reduction on the rates of febrile nonhemolytic transfusion reactions to platelet concentrates and RBCTransfusion, 2004
- Prestorage WBC filtration of RBC units with soft‐shell filters: filtration performance and impact on RBCs during storage for 42 daysTransfusion, 2002
- WBC‐reduced platelet concentrates from pooled buffy coats in additive solution: an evaluation of in vitro and in vivo measuresTransfusion, 2001
- Preparation of Leukodepleted Platelet Concentrates from Pooled Buffy Coats: Prestorage Filtration with Autostop™BCVox Sanguinis, 1999
- Six filters for the removal of white cells from red cell concentrates, evaluated at 4°C and/or at room temperatureTransfusion, 1999
- Leukocyte Reduction and Ultraviolet B Irradiation of Platelets to Prevent Alloimmunization and Refractoriness to Platelet TransfusionsNew England Journal of Medicine, 1997
- Practical guidelines for process validation and process control of white cell‐reduced blood components: report of the Biomedical Excellence for Safer Transfusion (BEST) Working Party of the International Society of Blood Transfusion (ISBT)Transfusion, 1996
- The Role of the Plasma from Platelet Concentrates in Transfusion ReactionsNew England Journal of Medicine, 1994
- Prevention of alloimmunization in patients with acute leukemia by use of white cell‐reduced blood components—a randomized trialTransfusion, 1991
- PREVENTION OF PRIMARY CYTOMEGALOVIRUS INFECTION AFTER ALLOGENEIC BONE MARROW TRANSPLANTATION BY USING LEUKOCYTE-POOR RANDOM BLOOD PRODUCTS FROM CYTOMEGALOVIRUS-UNSCREENED BLOOD-BANK DONORSTransplantation, 1990