Cloning and expression of a novel rat GABAA receptor
- 27 March 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 246 (1-2), 145-148
- https://doi.org/10.1016/0014-5793(89)80271-6
Abstract
Two full-length cDNA clones encoding α- and β-subunits of a GABAA receptor have been isolated from a rat cerebral cortex cDNA library. The mature α-subunit protein consists of 428 amino acids with a calculated M r of 48680. This protein is highly homologous (∼99% amino acid identity) with the bovine brain α1-subunit receptor [(1988) Nature 335, 76–79]. The mature rat β-subunit receptor is a 448 amino acid polypeptide and shares ∼ 80% amino acid identity with the previously characterized bovine GABAA receptor β-subunit [(1987) Nature 328, 221–227]. Co-expression of the cloned DNA in Xenopus oocytes produces a functional receptor and ion channel with pharmacological characteristics of a GABAA receptor. GABAA α- and β-subunit mRNA is detectable in the cortex, cerebellum and hippocampus.Keywords
This publication has 17 references indexed in Scilit:
- The mRNAs encoding GABAA/Benzodiazepine receptor subunits are localized in different cell populations of the bovine cerebellumNeuron, 1988
- Structural and functional basis for GABAA receptor heterogeneityNature, 1988
- The type I and type II γ-aminobutyric acid/benzodiazepine receptor: 1. Purification and two-dimensional electrophoretic analysis of the receptor from cortex and cerebellumBiochemical and Biophysical Research Communications, 1987
- Identification of a Family of Muscarinic Acetylcholine Receptor GenesScience, 1987
- Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-familyNature, 1987
- DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.Proceedings of the National Academy of Sciences, 1987
- [1] High-efficiency cloning of full-length cDNA; Construction and screening of cDNA expression libraries for mammalian cellsMethods in enzymology, 1987
- Vasopressin and oxytocin mRNAs in adrenalectomized and Brattleboro rats: analysis by quantitative in situ hybridization histochemistryMolecular Brain Research, 1986
- Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytesProceedings of the Royal Society of London. B. Biological Sciences, 1982
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977