Identification and Inhibitory Properties of Multifunctional Peptides from Pea Protein Hydrolysate
- 7 October 2010
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 58 (21), 11471-11476
- https://doi.org/10.1021/jf102538g
Abstract
Pea protein isolate was hydrolyzed with alcalase, and the hydrolysate passed through a 1 kDa cutoff ultrafiltration membrane. The permeate was freeze-dried and fractionated on a cationic solid-phase extraction (SPE) column. All fractions were tested for their inhibitory activities against angiotensin-converting enzyme (ACE), renin, and calmodulin-dependent phosphodiesterase 1 (CaMPDE). With the exception of the first eluted fraction, inhibitory properties of the SPE fractions against CaMPDE (but not ACE and renin) were directly related to cationic character (residence time on the column). However, the fraction that eluted with 1% ammonium hydroxide (SPE 1%) had the highest peptide yield and was subsequently fractionated using two consecutive rounds of reversed-phase high-performance liquid chromatography to obtain three peaks with major peptides identified as IR, KF, and EF by ultra performance liquid chromatography−tandem mass spectrometry. The three dipeptides showed weak inhibitory properties toward CaMPDE but strong inhibitions (IC50 values <25 mM) of ACE and renin. In general, the peptides had higher potency against ACE than against renin. It is indicated from our results that these peptides may be used as potential ingredients to formulate multifunctional food products and nutraceuticals.This publication has 38 references indexed in Scilit:
- Oyster (Crassostrea gigas) Hydrolysates Produced on a Plant Scale Have Antitumor Activity and Immunostimulating Effects in BALB/c MiceMarine Drugs, 2010
- Purification, Activity and Sequence of Angiotensin I Converting Enzyme Inhibitory Peptide from Alcalase Hydrolysate of Peanut FlourJournal of Agricultural and Food Chemistry, 2009
- Acute ingestion of a novel whey-derived peptide improves vascular endothelial responses in healthy individuals: a randomized, placebo controlled trialNutrition Journal, 2009
- Combination renin-angiotensin system blockade with the renin inhibitor aliskiren in hypertensionJournal of the Renin-Angiotensin-Aldosterone System, 2009
- Production of Lactoferricin and Other Cationic Peptides from Food Grade Bovine Lactoferrin with Various Iron Saturation LevelsJournal of Agricultural and Food Chemistry, 2006
- Novel angiotensin I-converting enzyme inhibitory peptides isolated from Alcalase hydrolysate of mung bean proteinJournal of Peptide Science, 2006
- Structural modulation of calmodulin and calmodulin-dependent protein kinase II by pea protein hydrolysatesInternational Journal of Food Sciences and Nutrition, 2006
- Kinetics of the inhibition of calcium/calmodulin-dependent protein kinase II by pea protein-derived peptidesThe Journal of Nutritional Biochemistry, 2005
- Renin inhibitory peptides. Incorporation of polar, hydrophilic end groups into an active renin inhibitory peptide template and their evaluation in a human renin-infused rat model and in conscious sodium-depleted monkeysJournal of Medicinal Chemistry, 1991
- Highly potent and specific inhibitors of human reninBiochemical and Biophysical Research Communications, 1984