Multifunctional Antimicrobial Nanofiber Dressings Containing ε-Polylysine for the Eradication of Bacterial Bioburden and Promotion of Wound Healing in Critically Colonized Wounds
- 8 April 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Applied Materials & Interfaces
- Vol. 12 (14), 15989-16005
- https://doi.org/10.1021/acsami.9b21683
Abstract
Bacterial colonization of acute and chronic wounds is often associated with delayed wound healing and prolonged hospitalization. The rise of multi-drug resistant bacteria and the poor biocompatibility of topical antimicrobials warrant safe and effective antimicrobials. Antimicrobial agents that target microbial membranes without interfering with the mammalian cell proliferation and migration hold great promise in the treatment of traumatic wounds. This article reports the utility of superhydrophilic electrospun gelatin nanofiber dressings (NFDs) containing a broad-spectrum antimicrobial polymer, epsilon-polylysine (epsilon PL), crosslinked by polydopamine (pDA) for treating second-degree burns. In a porcine model of partial thickness burns, NFDs promoted wound closure and reduced hypertrophic scarring compared to untreated burns. Analysis of NFDs in contact with the burns indicated that the dressings trap early colonizers and elicit bactericidal activity, thus creating a sterile wound bed for fibroblasts migration and re-epithelialization. In support of these observations, in porcine models of Pseudomonas aeruginosa and Staphylococcus aureus colonized partial thickness burns, NFDs decreased bacterial bioburden and promoted wound closure and re-epithelialization. NFDs displayed superior clinical outcome than standard-of-care silver dressings. The excellent biocompatibility and antimicrobial efficacy of the newly developed dressings in preclinical models demonstrate its potential for clinical use to manage infected wounds without compromising tissue regeneration.Keywords
Funding Information
- Singapore Eye Research Institute (NHIC-I2D-1712194, R1181/83/2014)
- Agency for Science, Technology and Research (H17/01/a0/0K9)
- National Medical Research Council (NMRC/CBRG/0048/2013, NMRC/CG/M010/2017_SERI, NMRC/TCR/008-SERI/2013)
- Ministerio de Econom?a y Competitividad (SAF2016-76150-R)
- National University of Singapore (Duke-NUS-KBrFA/2018/0007)
- Lee Kong Chian School of Medicine, Nanyang Technological University Singapore (L0494003)
This publication has 66 references indexed in Scilit:
- A systematic review of silver-containing dressings and topical silver agents (used with dressings) for burn woundsBurns, 2012
- Designing antimicrobial peptides: form follows functionNature Reviews Drug Discovery, 2011
- Review of resistance of ocular isolates of Pseudomonas aeruginosa and staphylococci from keratitis to ciprofloxacin, gentamicin and cephalosporinsClinical and Experimental Optometry, 2011
- Incidence and bacteriology of burn infections at a military burn centerBurns, 2010
- Mortality and causes of death in a burn centreBurns, 2008
- A porcine deep dermal partial thickness burn model with hypertrophic scarringBurns, 2006
- Infection and the Chronic WoundAdvances in Skin & Wound Care, 2005
- Outcome measures in burn care: Is mortality dead?Burns, 2004
- Mutation of lasA and lasB reduces Pseudomonas aeruginosa invasion of epithelial cellsMicrobiology, 2003
- Bacteriology of burnsBurns, 1998