Alterations in a yeast protein resembling HIV Tat-binding protein relieve requirement for an acidic activation domain in GAL4
- 1 June 1992
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 357 (6380), 698-700
- https://doi.org/10.1038/357698a0
Abstract
The acidic transcriptional activation motif functions in all eukaryotes, which suggests that it makes contact with some universal component of the transcriptional apparatus. Transcriptional activation by the yeast regulatory protein GAL4 requires an acidic region at its carboxyl terminus. Here we implement a selection scheme to determine whether GAL4 can still function when this C-terminal domain has been deleted. It can, when accompanied by a mutation in the SUG1 gene which is an essential gene in yeast. Analysis of mutant SUG1 in combination with various alleles of GAL4 indicates that SUG1 acts through a transcriptional pathway that depends on GAL4, but requires a region of GAL4 other than the C-terminal acidic activation domain. The predicted amino-acid sequence of SUG1 closely resembles that of two human proteins, TBP1 and MSS1, which modulate expression mediated by the human immunodeficiency virus tat gene.Keywords
This publication has 15 references indexed in Scilit:
- GAL11P: A yeast mutation that potentiates the effect of weak GAL4-derived activatorsCell, 1990
- Phosphorylated forms of GAL4 are correlated with ability to activate transcription.Molecular and Cellular Biology, 1990
- Yeast Gal11 protein mediates the transcriptional activation signal of two different transacting factors, Gal4 and general regulatory factor I/repressor/activator site binding protein 1/translation upstream factor.Proceedings of the National Academy of Sciences of the United States of America, 1990
- GAL11 protein, an auxiliary transcription activator for genes encoding galactose-metabolizing enzymes in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1988
- Yeast activators stimulate plant gene expressionNature, 1988
- GAL4 activates transcription in DrosophilaNature, 1988
- GAL4 activates gene expression in mammalian cellsCell, 1988
- The yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans-activatorCell, 1988
- Mutants of GAL4 protein altered in an activation functionCell, 1987
- Analysis of theKluyveromyces lactispositive regulatory geneLAC9reveals functional homology to, but sequence divergence from, theSaccharomyces cerevisiae GALAgeneNucleic Acids Research, 1986