Development of input connections in neural cultures
- 16 September 2008
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (37), 13758-13763
- https://doi.org/10.1073/pnas.0707492105
Abstract
We introduce an approach for the quantitative assessment of the connectivity in neuronal cultures, based on the statistical mechanics of percolation on a graph. This allows us to monitor the development of the culture and to see the emergence of connectivity in the network. The culture becomes fully connected at a time equivalent to the expected time of birth. The spontaneous bursting activity that characterizes cultures develops in parallel with the connectivity. The average number of inputs per neuron can be quantitatively determined in units of m(0), the number of activated inputs needed to excite the neuron. For m(0) similar or equal to 15 we find that hippocampal neurons have on average approximate to 60-120 inputs, whereas cortical neurons have approximate to 75-150, depending on neuronal density. The ratio of excitatory to inhibitory neurons is determined by using the GABA(A) antagonist bicuculine. This ratio changes during development and reaches the final value at day 7-8, coinciding with the expected time of the GABA switch. For hippocampal cultures the inhibitory cells comprise approximate to 30% of the neurons in the culture whereas for cortical cultures they are approximate to 20%. Such detailed global information on the connectivity of networks in neuronal cultures is at present inaccessible by any electrophysiological or other technique.Keywords
This publication has 26 references indexed in Scilit:
- Leader neurons in population bursts of 2D living neural networksNew Journal of Physics, 2008
- The physics of living neural networksPhysics Reports, 2007
- Identification and Dynamics of Spontaneous Burst Initiation Zones in Unidimensional Neuronal CulturesJournal of Neurophysiology, 2007
- In vivo imaging of the diseased nervous systemNature Reviews Neuroscience, 2006
- Transport of Information along Unidimensional Layered Networks of Dissociated Hippocampal Neurons and Implications for Rate CodingJournal of Neuroscience, 2006
- Trophic actions of GABA on neuronal developmentTrends in Neurosciences, 2005
- Excitatory actions of gaba during development: the nature of the nurtureNature Reviews Neuroscience, 2002
- Quantitative Distribution of GABA-immunopositive and-immunonegative Neurons and Synapses in the Monkey Striate Cortex (Area 17)Cerebral Cortex, 1992
- A laminar analysis of the number of neurons, glia, and synapses in the visual cortex (area 17) of adult macaque monkeysJournal of Comparative Neurology, 1982
- Development of the hippocampal region in the rat I. Neurogenesis examined with 3H‐thymidine autoradiographyJournal of Comparative Neurology, 1980