Salt Stress in Desulfovibrio vulgaris Hildenborough: an Integrated Genomics Approach
Open Access
- 1 June 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 188 (11), 4068-4078
- https://doi.org/10.1128/jb.01921-05
Abstract
The ability of Desulfovibrio vulgaris Hildenborough to reduce, and therefore contain, toxic and radioactive metal waste has made all factors that affect the physiology of this organism of great interest. Increased salinity is an important and frequent fluctuation faced by D. vulgaris in its natural habitat. In liquid culture, exposure to excess salt resulted in striking elongation of D. vulgaris cells. Using data from transcriptomics, proteomics, metabolite assays, phospholipid fatty acid profiling, and electron microscopy, we used a systems approach to explore the effects of excess NaCl on D. vulgaris . In this study we demonstrated that import of osmoprotectants, such as glycine betaine and ectoine, is the primary mechanism used by D. vulgaris to counter hyperionic stress. Several efflux systems were also highly up-regulated, as was the ATP synthesis pathway. Increases in the levels of both RNA and DNA helicases suggested that salt stress affected the stability of nucleic acid base pairing. An overall increase in the level of branched fatty acids indicated that there were changes in cell wall fluidity. The immediate response to salt stress included up-regulation of chemotaxis genes, although flagellar biosynthesis was down-regulated. Other down-regulated systems included lactate uptake permeases and ABC transport systems. The results of an extensive NaCl stress analysis were compared with microarray data from a KCl stress analysis, and unlike many other bacteria, D. vulgaris responded similarly to the two stresses. Integration of data from multiple methods allowed us to develop a conceptual model for the salt stress response in D. vulgaris that can be compared to those in other microorganisms.Keywords
This publication has 60 references indexed in Scilit:
- The role of potassium as an ionic signal in the regulation of cyanobacterium Nostoc muscorum response to salinity and osmotic stressJournal of Basic Microbiology, 2005
- Na+-dependent K+ Uptake Ktr System from the Cyanobacterium Synechocystis sp. PCC 6803 and Its Role in the Early Phases of Cell Adaptation to Hyperosmotic ShockOnline Journal of Public Health Informatics, 2004
- The RpoS Sigma Factor in the Dissimilatory Fe(III)-Reducing Bacterium Geobacter sulfurreducensJournal of Bacteriology, 2004
- Protein Displacement by DExH/D "RNA Helicases" Without Duplex UnwindingScience, 2004
- Genome-Wide Transcriptional Profiling Analysis of Adaptation ofBacillus subtilisto High SalinityJournal of Bacteriology, 2003
- Synchrotron infrared spectromicroscopy as a novel bioanalytical microprobe for individual living cells: cytotoxicity considerationsJournal of Biomedical Optics, 2002
- Characterization of Glycine Sarcosine N -Methyltransferase and Sarcosine Dimethylglycine N -MethyltransferaseApplied and Environmental Microbiology, 2001
- NADH:Ubiquinone Oxidoreductase of Vibrio alginolyticus: Purification, Properties, and Reconstitution of the Na+ PumpBiochemistry, 1996
- Monitoring of aVibrio natriegensandDesulfovibrio vulgarismarine aerobic biofilm on a stainless steel surface in a laboratory tubular flow systemJournal of Applied Bacteriology, 1991
- Growth of Desulfovibrio species on Hydrogen and Sulphate as Sole Energy SourceMicrobiology, 1981