Establishment of a monoclonal anti‐pan HLA class I antibody suitable for immunostaining of formalin‐fixed tissue: Unusually high frequency of down‐regulation in breast cancer tissues
- 16 March 2012
- journal article
- Published by Wiley in Pathology International
- Vol. 62 (5), 303-308
- https://doi.org/10.1111/j.1440-1827.2012.02789.x
Abstract
A novel monoclonal anti-pan human leukocyte antigen (HLA) class I heavy chain antibody, EMR8-5, was established. It could detect HLA-A, -B, and -C antigens in formalin-fixed paraffin embedded tissues. By immunohistochemical staining using the EMR8-5 antibody, various cancer tissues from 246 cases were examined for HLA class I expression. It was found that HLA class I expression was decreased in 20% to 42% of the cases of lung cancer, hepatocellular carcinoma, colon cancer, renal cell carcinoma, and urothelial carcinoma. In contrast, 85% of breast cancer cases had loss of or decreased HLA class I expression. Of the 35 breast cancer cases that had decreased HLA class I heavy chain expression, 33 (94%) also had decreased beta2-microglobulin expression detected by immunohistochemical staining. It was suggested that HLA class I down-regulation might be a common characteristic of breast cancer mostly caused by the down-regulation of beta2-microglobulin expression.Keywords
This publication has 22 references indexed in Scilit:
- Down-regulation of Human Leukocyte Antigen class I heavy chain in tumors is associated with a poor prognosis in advanced esophageal cancer patientsInternational Journal of Oncology, 2011
- Allele frequency net: a database and online repository for immune gene frequencies in worldwide populationsNucleic Acids Research, 2010
- Analysis and clinical relevance of human leukocyte antigen class I, heavy chain, and β2-microglobulin downregulation in breast cancerHuman Immunology, 2009
- Downregulation of HLA Class I molecules in the tumour is associated with a poor prognosis in patients with oesophageal squamous cell carcinomaBritish Journal of Cancer, 2008
- Mechanisms of immune evasion of human neuroblastomaCancer Letters, 2005
- Peptide-based vaccination for colorectal cancerExpert Opinion on Biological Therapy, 2005
- Selective monomorphic and polymorphic HLA class I antigenic determinant loss in surgically removed melanoma lesionsTissue Antigens, 2005
- Cytotoxicity of Histocompatibility Leukocyte Antigen–DR8–restricted CD4+ Killer T Cells against Human Autologous Squamous Cell CarcinomaJapanese Journal of Cancer Research, 1997
- HLA antigen expression in routinely processed head and neck squamous cell carcinoma primary lesions of different sitesInternational Journal of Cancer, 1991
- HLA-A- and HLA-B-specific monoclonal antibodies reactive with free heavy chains in Western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopyInternational Immunology, 1990